fa Morphometry Morphometry Original Original <strong>Purpose:</strong> The objective of this study was to evaluate the effects of myo-inositol alone or in combination with MEM vitamins on embryo development in sanjabi sheep.<strong>Materials and Methods</strong>: Sheep Cumulus Oocytes Complexes (COCs) were matured in vitro at 39&ordm;C, in humidified 5% CO2 atmosphere for 22-24 h. There were three treatments, culture in synthetic oviductal fluid medium (treatment I), culture in synthetic oviductal fluid medium supplemented with1&times;MEM vitamins (treatment II), culture in synthetic oviductal fluid medium supplemented with myo-inositol (treatment III), COCs were then fertilized and cultured in vitro for 8 days when the ratios of in vitro embryo development of the hatched blastocysts were assessed and compared with the control group.<strong>Results</strong>: The presence of myo-inpsitol significantly improved overall morula rates (34.39%) than that of control group (24.18%), but there was no difference between myo-inositol and 1&times;MEM vitamins in the percentage of embryos successfully developing to the morula stage (p<0.05). The addition of myo-inositol improved the mean blastocyst formation compared to the oocytes matured in medium containing no MEM (control) or 1&times;MEM vitamins (32.63, 13.96, and 21.06% respectively). However the mean percentage of cleavage rate was not substantialy different between treatments. <strong>Conclusion</strong>: These results suggest that adding myo-inositol to SOF medium be more beneficial for subsequent sheep embryonic development. Myo–Inositol, In Vitro Fertilization, Embryo Development, Sanjabi Sheep 0 0 http://anatomyjournal.ir/browse.php?a_code=A-10-304-217&slc_lang=fa&sid=1 Farokh Kafilzadeh 10031947532846006114 10031947532846006114 Yes Hamed Karami Shabankareh 10031947532846006115 10031947532846006115 No Leila Soltani 10031947532846006116 10031947532846006116 No Vida Kazemine Jasemi 10031947532846006117 10031947532846006117 No